| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides
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| Autores: |
Rodrigues,José Ariévilo Gurgel
Vanderlei,Edfranck de Sousa Oliveira
Bessa,Érika Façanha
Magalhães,Francisco de Araújo
Paula,Regina Célia Monteiro de
Lima,Vilma
Benevides,Norma Maria Barros
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| Data: |
2011-08-01
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| Ano: |
2011
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| Palavras-chave: |
Marine algae
Sulfated macromolecules
Chemical analysis
APTT test
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| Resumo: |
The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl, respectively. Only fraction F II was active (24.62 IU mg-1), with high sulfate content (23.79%) and number of molecular mass peaks. Therefore, the APTT of a fraction isolated from C. cupressoides was less potent than HEP.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400007
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132011000400007
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.54 n.4 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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